HEPES is White crystalline powder, while it's Molecular Formula is C8H18N2O4S. White crystalline powder HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

What is the CAS number for HEPES?

The CAS number of HEPES is 7365-45-9.

What is HEPES (7365-45-9) used for?

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

HEPES is White crystalline powder, while it's Molecular Formula is C8H18N2O4S. White crystalline powder HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

What is the CAS number for HEPES?

The CAS number of HEPES is 7365-45-9.

What is HEPES (7365-45-9) used for?

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

7365-45-9

HEPES

别名：

CasNo.： 7365-45-9

分子式： C₈H₁₈N₂O₄S

分子量： 238.308

储存条件：

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中文名称： HEPES

英文名称： HEPES

CasNo.： 7365-45-9

分子式： C₈H₁₈N₂O₄S

分子量： 238.308

Quality Factory Supply 99% Pure HEPES 7365-45-9 with Efficient Delivery

Molecular Formula:C8H18N2O4S
Molecular Weight:238.308
Appearance/Colour:White crystalline powder
Vapor Pressure:0Pa at 25℃
Melting Point:234-238 °C
Refractive Index:n20/D 1.339
Boiling Point:408℃[at 101 325 Pa]
PKA:7.5(at 25℃)
PSA：89.46000
Density:1.325 g/cm³
LogP:-0.55930

HEPES(Cas 7365-45-9) Usage

Biological Activity	HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Purification Methods	Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
Definition	ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General Description	HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

7365-45-9 Relevant articles

Preparation method of piperazine ethanesulfonic acid derivative (by machine translation)

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Paragraph 0048-0087, (2020/02/06)

The reaction is carried out in such a ma...

Method for preparing high-purity 4-hydroxyethylpiperazine ethanesulfonic acid

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Paragraph 0034; 0035; 0047, (2017/07/19)

The invention belongs to the technical f...

High purity 4 - hydroxyethyl piperazine b sulfonic acid

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Paragraph 0053; 0054; 0064; 0065, (2017/09/26)

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COAGULATION FACTOR VII POLYPEPTIDES

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Page/Page column, (2015/04/21)

The present invention relates to modifie...

7365-45-9 Process route

: 103-76-4
1-(2-hydroxyethyl)piperazine

: 1562-00-1
sodium 2-hydroxyethanesulfonate

: 7365-45-9
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid

Conditions

Conditions	Yield
1-(2-hydroxyethyl)piperazine; sodium 2-hydroxyethanesulfonate; In methanol; at 50 ℃; for 5.5h; With 5percent by weight of raffinate; In water; Solvent; Temperature;	85.6%